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KMID : 1098420060140020087
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Korean Journal of Medicinal Crop Science
2006 Volume.14 No. 2 p.87 ~ p.91
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Plant Regeneration Via Organogenesis on Petiole of Centella asiatica (L.) Urban
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Choi Kyung-Mi
Hwang Sung-Jin
Chung Sang-Jin
Hwang Baik
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Abstract
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An efficient plant regeneration of C. asiatica was achieved from organogenesis using petiole explants of in vitro plantlet on MS basal medium controled with different plant growth regulators (NAA,2,4-D, IAA kinetin, and BA). Best results that 50%, efficiency of regeneration per explant for regeneration were obtained with IAA 17.13;{mu}M and BA 8.9;{mu}M. Formation of adventitious shoots via organogenesis from the petiole explant was verified by histological sectioning of plantlets. Regenerated plants were transplanted into soil.
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KEYWORD
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Centella asiatica, adventitious shoot, organogenesis, histological section
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